Thursday, September 8

Oil Immersion

Yesterday in my Microbiology Lab we learned how to do Oil Immersion, which is a process that is used in microscopy to increase the resolution at much higher magnification. I though it was a pretty clever way to increase resolution. Here is a cool diagram that shows the process
Principle of immersion microscopy. Path of rays with immersion medium (yellow) (left half) and without (right half). Rays (black) coming from the object (red) at a certain angle and going through the coverslip (orange, as the slide at the bottom) can enter the objective (dark blue) only when immersion is used. Otherwise, the refraction at the coverslip - air interface causes the ray to miss the objective and its information is lost.